Optimal maternal nutrition during the first 1,000 days is important to improve nutritional status and health outcomes for women and their infants. This updated guidance, building on the Maternal Nutrition Operational Guidance: Program Considerations for Low- and Middle-Income Countries, provides step-by-step recommendations to add and strengthen maternal nutrition components in programs or services delivered by the health system, including actions to strengthen the overall health system and the enabling environment. This guide also incorporates a program planning process and includes additional information on collecting and synthesizing data.