This analysis presents the status of micronutrient deficiencies among children under 5 years of age and women of reproductive age in Guatemala, with an emphasis on iron, zinc, vitamin B12, folate, vitamin A, and iodine. The report provides evidence for the need to develop and update policy and technical guidelines in Guatemala to address micronutrient deficiencies as a public health concern, and within the context of Guatemala’s national strategy for the prevention of chronic malnutrition.